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Easy Cell Transfection Kit


Category

mRNA Raw Material

Kits


Art.No.:

Refer to below


Product Name:

Easy Cell Transfection Kit


Purity

None

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Technical Parameter

Technical Parameter

Product Description

Easy Cell Transfection Kit is a high-efficiency liposome transfection reagent, which has the characteristics of high efficiency, low toxicity, convenient operation and stability, and is widely used for mRNA and DNA transfection of various cell lines.

Unit Size

0.1ml

0.5ml

1.0ml

5*1.0ml

Catalog #

10114-0.1

10114-0.5

10114-1.0

10114-5.0

 

Product advantages

High transfection efficiency - ultra-high transfection efficiency can be achieved for common cell lines

Low toxicity - low cytotoxicity, maintaining excellent cell viability and morphology

Good repeatability - high experimental repeatability and strong operability

High cost performance - high transfection rate, low price, economical and practical

   

Transfection examples

1、Use Easy Cell Transfection Kit transfection reagent to transfect eGFP mRNA into Hela, 293T and other cell lines respectively. For the transfection effect, refer to Table 1 and Figure 2 to Figure 5.

Table 1 Evaluation of some transfection effects of Easy Cell Transfection Kit

Cell lines

Transfection effect

Hela

++++

293T

+++++

U251

++++

Jurkat

++++

Note: “+” indicates the transfection effect. The more “+”s, the better the transfection effect.

Figure 2 Actual picture after transfection of eGFP mRNA into Hela cells using Easy Cell Transfection Kit

 

Figure 3 Actual pictures after transfecting eGFP mRNA into 293T cells using Easy Cell Transfection Kit

 

Figure 4 Actual pictures after transfection of eGFP mRNA into U251 cells using Easy Cell Transfection Kit

 

Figure 5 Actual picture after transfection of eGFP mRNA into Jrukat cells using Easy Cell Transfection Kit

 

2、Use Easy Cell Transfection Kit transfection reagent to transfect 293T cells with two different sizes of target mRNA (mRNA1: ~1000 bp, mRNA2: ~3000 bp). After 24 hours of transfection, take the cells for WB detection of the target protein. The results are shown in Figure 6.

 

Figure 6 WB detection results after transfection of target mRNA using Easy Cell Transfection Kit

 

3、Use the Easy Cell Transfection Kit transfection reagent to transfect the pCMV-N-EGFP (green fluorescent protein) plasmid into the 293T cell line. Refer to Figure 6 for the transfection effect.

Figure 7 Actual picture of pCMV-N-EGFP plasmid transfected into 293T cells using Easy Cell Transfection Kit

 

The above data shows that using the Easy Cell Transfection Kit transfection reagent to transfect mRNA/plasmid into a variety of adherent/suspended cells can achieve effective transfection with high transfection efficiency and low cytotoxicity!

 

 

 

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