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mRNA/LNP detection service

As a global leading mRNA overall solution provider, Synthgene can not only provide high-quality mRNA raw materials, but also provide mRNA stock solution and mRNA-LNP comprehensive detection services, which supports mRNA vaccines/drugs development and production!

 

Synthgene can provide multi-dimensional mRNA/LNP detection services, including purity, integrity, capping rate, Poly A , encapsulation rate, PDI detection, etc, which comprehensively evaluates product quality, safety and biological activity.

mRNA detection service

Detection item

Detection method

Appearance

Visual method

PH

ChP 0631

mRNA sequence identity confirmation

RT-PCR

mRNA concentration

UV

mRNA Integrity

CGE/AGE

mRNA Purity(percentage of fragment mRNA)

RP-HPLC

mRNA Purity(aggregate quantitation)

SEC-HPLC

Capping rate

LC-MS

PolyA tail length

LC-MS

dsRNA

ELISA

DNA residue

qPCR

Protein residue

Nano Orange/ELISA

Plasmid purity

HPLC

Plasmid template purity

HPLC/CGE

Nucleotides and cap analogs residue

HPLC

Endotoxin

SG/DCR
mRNA-LNP detection service
Appearance Visual method
PH ChP 0631
Osmotic pressure ChP 0632
mRNA sequence identity confirmation RT-PCR
Identification and quantification of LNP components HPLC-CAD
mRNA Integrity CGE/SEC
mRNA Purity(percentage of fragment mRNA) RP-HPLC
mRNA Purity(aggregate quantitation) SEC-HPLC
Encapsulation mRNA content/Encapsulation rate RiboGreen assay
Lipid content HPLC-CAD
PS & PDI DLS
Zeta ELS
Subvisible particles Light Obscuration Particles Count Test/Microscopic Particles Count Test)
Residual solvent USP<467>、ChP 0861
Endotoxin SG/DCR
Biological activity Flow/ELISA